Objectives: To search for susceptibility genes within the large genetic interval (20 cM ) identified by linkage we performed association analysis of selected candidate genes.
Methods: 350 multiplex autistic families were subjected to transmission disequilibrium test (TDT) analysis.
Results: We detected an association with ~220 kb interval situated right under the linkage peak. Signals arose from multiple SNPs comprising several haploblocks with the largest one extending over 100 kb. The associated region contains 5 protein-coding genes, and 4 of which are expressed in the brain. At least 3 of 5 candidate genes are imprinted in human and mouse. Mutations affecting coding sequences/splice sites of 7q32 candidate genes have been searched by sequencing of 100 autists and 100 control individuals.
Conclusions: We identified a new candidate region for autism on 7q32. The associated locus contains several imprinted genes. The involvement of epigenetic component - altered imprinting in autism may explain increasing incidence of the disorder.