Objectives: This study investigated the effect of rs1611115 on DBH promoter function using a reporter assay system.
Methods: Inserts representing each allele at rs1611115 were inserted into the pGL3 basic reporter vector (,). Each construct was then transfected into two cell lines – CHO K1 and SH SY-5Y. Differences in promoter activity between alleles was then determined using a luciferase reporter assay system (, ).
In the non-neuronal CHO K1 cell line, neither reporter construct increased relative expression levels of the luciferase reporter gene. In the neuronal SH SY-5Y cell line, both constructs increased gene expression beyond background levels. Additionally, significant allelelic differences in relative expression levels were observed (p=0.0049).
This finding confirms at the molecular level previous reports that variation at rs1611115 affects DBH promoter efficiency.