Friday, May 21, 2010
Franklin Hall B Level 4 (Philadelphia Marriott Downtown)
Background: Autism is a developmental disorder. Accumulating evidence suggests that oxidative stress may provide a link between susceptibility genes and pre- and post-natal environmental stressors in the pathophysiology of autism. Abnormal lipid oxidation, protein oxidation and energy metabolites (pyruvate and lactate) have been implicated in autism and other neuropsychiatric disorders such as depression and ADHD. No such biochemical data is available for normal and autistic children in Sultanate of Oman.
Objectives: This study was aimed to compare the status of circulatory reactive nitrogen species (RNS), protein oxidation, lipid oxidation and pyruvate in normal and Omani autistic children.
Methods: The blood/plasma samples were collected from SQU hospital, Oman from autistic and control children. Protein and lipid oxidation was assessed in the plasma by quantitation of protein carbonyls and TBARS using ELISA kits (Cayman Inc) respectively. Pyruvate was measured by using pyruvate assay kit (Bio vision). RNS was measured by using Nitric oxide fluorometric assay kit from Bio vision.
Results: Altered protein carbonyls, lipid oxidation, RNS and pyruvate were found in Omani autistic children as compared with controls.
Conclusions: This is the first study in Omani autistic children and the outcome of this study may give a lead to develop a novel biomarker for early detection of autism. Also this study will give the relationship between oxidative stress and the pathophysiology of autism.