Sexual Dimorphism of Regulatory Small Non-Coding RNA in the Superior Temporal Gyrus Brain Region of Autism Spectrum Disorders: A Pilot Study
Objectives: To assess sncRNA expression in STS and PAC in postmortem male and female human brains of ASD compared to TD controls.
Methods: Affymetrix miRNA 3.0 arrays were run on 34 samples (5 ASD Female, 5 ASD Male, 2 TD Female, 6 TD Male; two brain regions – STS, PAC; 4-58 years of age; two of the 18 subjects had only one brain region available). ANOVA was used to identify sexually dimorphic differentially expressed sncRNA (p<0.005, |fold-change|>1.2). REML variance estimate suitable for unbalanced designs was used. To account for normal sexual dimorphism, we compared ASD female to TD female and ASD male to TD male, and overlapped the differentially expressed sncRNA to identify sexually dimorphic sncRNA expression specifically in ASD (Fig.1).
Results: We found sexually dimorphic sncRNA expression in the ASD postmortem brain, with a higher number of sncRNA being dysregulated in females compared to males (Fig.1). There were 27 sexually dimorphic sncRNA in ASD STS (7 in male and 20 in female), 9 in ASD PAC (2 in male, 7 in female), and 71 regionally dysregulated (between STS and PAC) (31 in male, 39 in female, and 2 common) (Fig. 2). The predicted mRNA targets of the mature miRNAs are over-represented in different pathways in male and female in STS and PAC. Axonal Guidance Signaling was the most over-represented canonical pathway in the regionally dysregulated (STS vs PAC) mature miRNAs both in male (FDR-corrected p=3.1E-12) and female (FDR-corrected p=2.4E-09). However, most of the predicted dysregulated target mRNAs were different in males compared to females. A number of predicted targets have been implicated in ASD, such as MET (in female), NTRK3 (in male), and SHANK2 (in both).
Conclusions: The sexually dimorphic sncRNAs in male and female ASD brains likely contribute to aberrant development and function of STS and PAC and likely contribute to some of the sexually dimorphic features of ASD. Future studies will need to confirm these findings.