Measurement of Multiple Radical Scavenging Activity As a Diagnostic Method for Autism Spectrum Disorder in Children

Thursday, May 11, 2017: 5:30 PM-7:00 PM
Golden Gate Ballroom (Marriott Marquis Hotel)
H. Matsuzaki1 and A. Hirayama2, (1)Research Center for Child Ment, University of Fukui, Eiheiji-cho, Fukui, Japan, (2)Center for Integrative Medicine, Tsukuba University of Technology, Tsukuba City, Ibaraki, Japan
Background:  Oxidative stress plays a central role in the pathogenesis of autism spectrum disorders (ASD). Though early interventions are important, ASDs are rarely diagnosed in toddlers, because diagnosis is based deeply on psychological assessment and observation.

Objectives:  We investigated the availability of the measurements of multiple free radical scavenging activity (MULTIS) as a novel diagnostic tool for ASD children.

Methods:  Serum samples from ASD children and neurotypical children (NT) were analyzed under informed consents. This study enrolled 23 children (3.9±0.8 y.o.) with ASD recruited at the Miyagi children's hospital (Sendai, Japan) and 34 age-matched NT recruited by advertisement. DSM-IV-TR diagnosis of autistic disorder was made for all subjects. Fasting human blood samples were collected by venipuncture in a sitting position with a tourniquet from all participants for both groups who are Japanese and drug-naïve. In MULTIS method, serum scavenging-activity profile against 5 reactive oxygen species, namely .OH, O2.-, RO., ROO. and 1O2 were measured using spin traps CYPMPO and TMPO (Oowada S et al., J Clin Biochem Nutr. 2012;51:117-21.).

Results:  Scavenging activity against was significantly decreased in ASD group (approximately 55% of NT group mean value). Contrary to .OH, scavenging activities against O2.- and RO. were significantly increased in ASD group (approximately 120% and 157% of NT group mean values, respectively). No differences were observed in ROO. and 1O2 among the two groups. When diagnostic criteria for ASD was made using .OH, O2.- and RO. scavenging activities, the calculated sensitivity, specificity and positive likelihood ratio were 65%, 93% and 9.78, respectively. Moreover, when the data of ROO. and 1O2 were added, the positive likelihood ratio increased to 22.17.

Conclusions:  Measurements of serum multiple free radical scavenging activity by MULTIS method could be a very powerful non-behavioral tool for diagnosis of ASD children.