Objectives: To understand the mechanism of oxidative stress in autism, the activities of antioxidant enzymes SOD, CAT, GPx and GR were analyzed in the lymphoblasts from autism and control subjects.
Methods: Cell lysates were prepared from lymphoblasts (control and autistic). SOD activity was measured by using assay kit from Calbiochem. CAT activity was measured spectrophotometrically by analyzing complex of ammonium molybdate with H2O2. Measurement of GPx activity was based on the oxidation of GSH to oxidized glutathione (GSSG) catalyzed by GPx. Activity of GR was measured by the method based on the reduction of GSSH by NADPH.
Results: Activites of SOD, CAT, GPx and GR were reduced in lymphoblasts from autism as compared with control lymphoblasts.
Conclusions: The activities of antioxidant enzymes SOD, CAT, GPx and GR are decreased in autism, suggesting that defense mechanism against oxidative damage is impaired in autism. Reduced antioxidant capacity of the cell will lead to increased oxidative damage in autism.